目的研究楚能Q-BIT光量子能量芯片对小鼠Lewis肺癌的预防作用的预防作用,为该楚能Q-BIT光量子能量芯片的使用提供药效学研究的试验资料。方法选择50只雌性C57BL/6小鼠,采用在小鼠腋下皮下注射0.2 ml/只浓度为2×1010 L-1的Lewis肺癌细胞,并将其分为空白对照组10只、模型对照组20只、芯片预防组进行体内抑瘤实验。试验组动物接种瘤细胞当天即进行芯片预防给药,5只/笼,芯片数量为6片/笼,即5只小鼠/290×178×160 mm空间/6片Q-BIT光量子能量芯片。其中阴性对照组和肿瘤模型对照组,动物接种肿瘤细胞后饲养在正常SPF环境中。芯片作用5周后处死实验动物,期间观察动物体重及肿瘤体积的变化,计算抑瘤率。并在实验结束时采集动物EDTA抗凝血,进行CD4+,CD8+和CD19+小鼠外周血T和B淋巴细胞分析免疫学指标测定。结果给药5周后,试验期间进行体重测定,组间比较采用t检验。阴性对照组的小鼠没有接种小鼠Lewis肺癌细胞,小鼠的体重呈现生理性的增长;与阴性对照组小鼠比较,阳性对照组的动物体重在接种肿瘤后第22天,体重增加有显著性差异(P<0.05),并且一直持续到试验结束;芯片预防组在接种肿瘤的第10天,芯片预防组与阳性对照组比较体重有显著性差异(P<0.05)。与阳性对照组比较,Q-BIT芯片预防组小鼠的肿瘤生长速度逐渐减缓,明显受到抑制,阳性对照组小鼠的肿瘤生长速度较快,生长曲线上升明显。对照组与芯片预防组组小鼠的肿瘤平均体积间差异没有有统计学意义(P>0.05)。阳性对照组组与芯片预防组小鼠的肿瘤平均瘤重之间差异亦没有有统计学意义(P>0.05),肿瘤抑制率为25%。与阴性对照组比较,芯片预防组CD4+T淋巴细胞百分数大于阳性对照组,即阴性对照组(64.3±1.5)>芯片预防组(61.8±4.6)>阳性对照组(60.5±4.3),差异没有统计学意义(P>0.05);CD8+T淋巴细胞百分比,阳性对照组与阴性对照组之间比较有统计学差异(P<0.05),但与试验组之间没有统计学差异(P>0.05);CD4/CD8比值以阳性对照组最高,与阴性对照组之间比较有统计学差异(P<0.05),但与试验组之间没有统计学差异(P>0.05);CD19+B淋巴细胞百分比,以阴性对照组较低(50.3±3.7)>芯片预防组(52.2±7.5)>阳性对照组(52.2±7.5),其差异没有统计学意义(P>0.05)。结论楚能Q-BIT光量子能量芯片虽然不能明显预防和抑制小鼠Lewis肺腺癌细胞在C57BL/6小鼠体内的生长,但能逐渐减缓肿瘤生长速度和提高机体免疫能力的趋势。 Objective To study the preventive effect of Chu-Q-BIT photon energy chip on the prevention of Lewis lung carcinoma in mice and provide the experimental data for the pharmacodynamic study of the Q-BIT photon quantum chip. Methods Fifty female C57BL / 6 mice were selected and subcutaneously injected with 0.2 ml Lewis lung carcinoma cells at a concentration of 2 × 1010 L-1 in the armpit of mice. The mice were divided into blank control group (n = 10), model control group 20, chip prevention group in vivo anti-tumor experiment. The test animals were inoculated with tumor cells on the same day for prophylactic administration of the chip, 5 / cage, the number of chips was 6 / cage, namely 5 mice / 290 × 178 × 160 mm space / 6 Q-BIT photon quantum chips. Among them, negative control group and tumor model control group, animals were inoculated with tumor cells in normal SPF environment. After 5 weeks of operation, the experimental animals were sacrificed and the changes of body weight and tumor volume were observed to calculate the tumor inhibition rate. At the end of the experiment, EDTA anticoagulant was collected and T / B lymphocyte analysis of CD4 +, CD8 + and CD19 + mice was performed. Results After 5 weeks of administration, body weight was measured during the test, t test was used to compare between groups. The mice in the negative control group were not inoculated with Lewis lung cancer cells in mice, and the body weight of the mice showed a physiological increase. Compared with the negative control mice, the body weight of the positive control group was significantly increased on the 22nd day after tumor inoculation (P <0.05), and continued until the end of the experiment. On the 10th day after tumor inoculation group, there was significant difference (P <0.05) between the preventive group and the positive control group. Compared with the positive control group, the tumor growth rate of Q-BIT prevention group was slowed down and obviously inhibited. The positive control group mice grew faster and the growth curve increased obviously. There was no significant difference in mean tumor volume between the control group and the chip prevention group (P> 0.05). There was no significant difference between the average weight of tumor in the positive control group and the chip prevention group (P> 0.05), and the tumor inhibition rate was 25%. Compared with the negative control group, the percentage of CD4 + T lymphocytes in the chip-preventive group was larger than that in the positive control group (64.3 ± 1.5)> 61.8 ± 4.6 in chip prevention group (60.5 ± 4.3), and no difference (P> 0.05). The percentage of CD8 + T lymphocytes in the positive control group and the negative control group were statistically significant (P <0.05), but there was no significant difference between the positive control group and the negative control group (P> 0.05) ). The ratio of CD4 / CD8 in the positive control group was the highest, which was significantly higher than that in the negative control group (P <0.05), but not significantly different from the experimental group (P> 0.05) (50.3 ± 3.7)> chip prevention group (52.2 ± 7.5)> positive control group (52.2 ± 7.5), the difference was not statistically significant (P> 0.05). CONCLUSION Chundu Q-BIT photon energy chip can not obviously prevent and inhibit the growth of mouse Lewis lung adenocarcinoma cells in C57BL / 6 mice, but can gradually reduce the tumor growth rate and improve the immune capacity.