Long non-coding RNAs (lncRNAs) are emerging as new regulators in the cancer paradigm,demonstrating potential roles in both oncogenic and tumor suppressive pathways.These novel genes are frequently aberrantly expressed in a variety of human cancers.However, the biological functions of the majority remain largely unknown.In this study, based on analysis of several malignant melanoma (MM) transcriptome datasets and our experimental data, we found that LSAT1 is weakly expressed in MM cells and attenuates tumor growth and metastasis of human melanoma cells.The expression of LSAT1 (uc031tha.1) was suppressed by hypoxic microenvironment.Moreover, the binding site of the master hypoxia-related transcription factor——STAT3 is located at the upstream of gene SAT1.There is a correlation between the expression of LSAT1 and the activity of STAT3 through bioinformatic analyses and experimental validation.We predict that LSAT1 is negatively regulated by STAT3.In summary, our findings indicate that LSAT 1 acts as a tumor suppressor in melanoma negatively regulated by the hypoxia-induced signaling.