Single cell sequencing,including the whole genome amplification-sequencing and the whole transcriptome sequencing,has been achieved recently.However,there are various technical challenges which may still hinder the application of single-cell studies in many fields.I am going to talk about two major technologies we recently developed,using microfluidic devices to achieve single cell whole genome sequencing and whole transcriptome sequencing.We developed a novel method that randomly distribute the whole genome from a single cell into a large number of emulsion droplets to perform multiple displacement amplification,resulting much evener amplification across the whole genome.This microfluidics-facilitated method enables simultaneous detection of copy number variations and single nucleotide variations in a individual cell,offering much finer resolution of CNV detection and significantly improved accuracy of SNV detection than existing single cell WGA methods.We also developed a microfluidic platform to perform single cell RNA-Seq sample prep.To probe subtle biological variation between samples with limiting amounts of RNA,more precise and sensitive methods are still required.We adapted a previously developed strategy for single-cell RNA-Seq that has shown promise for superior sensitivity and implemented the chemistry in a microfluidic platform for single-cell wholetranscriptome analysis.In this approach,single cells are captured and lysed in a microfluidic device.Microfluidic implementation increased mRNA detection sensitivity as well as improved measurement precision compared with tubebased protocols.We also quantified variation between and within different types of mouse embryonic cells and found that enhanced measurement precision,detection sensitivity,and experimental throughput aided the distinction between biological variability and technical noise.