Molecularrecognition is in full swing,and fluorescent probesin which are acclaimed among researchers and occupy a very important position in new materials and new energy sources[1-3].Among variant probes,the single identification is the majority,which makes the multi-identification outstanding that not only improves work efficiency,but also saves costs.A novel Schiff-baseprobe1wassynthesized and characterized,which exhibited different optical colors for Zn2+and Al3+at 535 nm and 518 nm respectively with “turn-on” fluorescence response(λex: 420 nm)in MeOH-H2O(4/1,v/v)medium and realized dual channel signal output for Al3+throughout replacement strategy.Probe1exhibitedan excellent sensitivity with detection limits of 0.04μM for Zn2+and 0.14 μM for Al3+,respectively.The binding mechanisms of 1towards Zn2+and Al3+ions were proposed to be C=N isomerizationand ICT process from fluorescence and UV-vis,1H NMR titration and DFT calculations(Fig.1).In addition,the 1-Zn2+and 1-Al3+systems could be further detectionPPi or PPi/F-through a “closed” fluorescence response with a detection limit of 0.47 μM,0.99 μM and 0.16 μM.Importantly,the probe appeared favorable membrane permeability and was able to sequentially monitor Zn2+,Al3+,PPi and F-in living PC12 cells.