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The object of this study was to investigate the effect of molybdenum on the development of mouse preimplantation embryos cultured in vitro.Zygotes were flushed from one outbred mouse strain (Kunming), and then were cultured in KSOM media containing 0, 5, 10, 20, 40, 80, 120, 160 μg/ml of molybdenum for 5 d until the mid-blastocyst stage.The addition of≤20 μg/ml molybdenum did not affect the blastocyst and birth rates.Molybdenum at doses of 40 μg/ml and higher significantly decreased the cleavage, blastocyst and birth rates, the average cell number, and significantly increased the proportion of degenerative blastocysts.120 μg/ml molybdenum inhibited the blastocysts to develop to birth.Further, 160 μg/ml molybdenum caused overall developmental arrest (up to 16-cells) of embryos and their massive degeneration.In conclusion, molybdenum negatively affected the development of embryos in a dose-dependent manner.With lower doses (≤20 μ,g/ml), mouse embryos were not apparently damaged.With very high doses (≥40 μg/ml), embryo quality was significantly decreased.This work may have some utility as one of toxicological data for risk assessment of molybdenum on the preimplantation embryo.