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The impact of welan gum on C3S hydration was examined to determine the retarding effect of welan gum on cement hydration.In this research, zeta potential analyzer and UV-visible adsorption spectrophotometer were used to study the surface electrochemical properties and adsorption characteristics of welan gum.Meanwhile,C3S hydration behaviors in the presence or absence of welan gum was researched by zeta potential analyzer,X-ray diffraction and field emission scanning electron microscopy.In addition to welan gum, the adsorption behavior and zeta potential inversion of C3S were observed as well as a rapid decrease of zeta potential and retarding effect on C3S system were also discussed.Besides, the reduction of nucleation rate and morphology change of Ca(OH)2 were demonstrated.Thus, through the adsorption effect, welan gum induces a retarding behavior of C3S hydration.