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To develop a reliable and rapid nucleic acid extraction method and detecting technique for certification and quarantine programs on plant materials, the extraction method based on silica-coated magnetic particles (SMPs method) and RT-qPCR assay were described.Arabis mosaic virus (ArMV), Lily symptomless virus (LSV), Hop stunt viroid (HSVd) and grape yellow speckle viroid 1 (GYSVd-1)was selected as research objects and the sequences including amplification regions of RT-qPCR were transcribed in vitro as standard RNA templates.The standard curves covered six or seven orders of magnitude with detection limits of 100 copies per each assay.