论文部分内容阅读
The red flour beetle,Tribolium castaneum(Coleoptera: Tenebrionidae),is a cosmopolitan pest of stored agricultural products worldwide.The pheromone 4,8-dimethyldecanal(DMD)released by feeding male contained four enantiomers and the relative ratio of four isomers(4R,8R):(4R,8S):(4S,8R):(4S,8S)at approximately 4:4:1:1.This paper reported that the biological response of adult T.castaneum to different blends of four enantiomers by a walking bioassay in a wind tunnel and electroantenngrams(EAG)with the four synthetic enantiomers.Compared with the commercial lure mixture,which is 1:1(4R,8R):(4R,8S),the blends that that mimics ratio that produced naturally by male,which is 4:4:1:1(4R,8R):(4R,8S):(4S,8R):(4S,8S)–DMD could elicited the highest walking response of mix sex of T.castaneum in wind tunnel bioassay in each dose from 0.01ng to 100ng.The individual four enantiomers(4R,8R)-,(4R,8S)-,(4S,8R)-,:(4S,8S)-DMD induced significant difference behavior response compared with hexane solvent control and(4R,8R)-DMD and commercial lure mixture had no significant difference behavior response at dose 100ng.The result of dose responses of T.castaneum indicated that the threshold of commercial lure was 10 ng.Compared with the mixture 4:1of(4R,8R)-and(4R.8S)-DMD,the blends mimicked commercial lure could elicit the significant difference walking response only at dose 50ng and the results confirmed that relative ratio of four enantiomers(4R,8R)-I:(4R,8S)-I:(4S,8R)-I:(4S,8S)-I of natural pheromone components at approximately 4:4:1:1 had highest bioassay response.EAG response to four individual enantiomers and their different blends confirmed that the blends mimicked natural pheromone components(mixture of 4:4:1:1(4R,8R)-I:(4R,8S)-I:(4S,8R)-I:(4S,8S)-I)had no significantly different with that of eluate from Porapak-Q-collected volatiles from feeding males either at 0.1ng or at 1.0ng.The lab.strain GA-1,originally field-collected in Georgia,USA and the KS strain,originally field-collected in Kansas,USA had no significant differences in response to the 4:4:1:1(4R,8R)-I:(4R,8S)-I:(4S,8R)-I:(4S,8S)-I synthetic blend and the natural volatiles collected on Porapak-Q from feeding males at 0.1ng.