【摘 要】
：
Regulated secretion of Human Amylin (hAmylin) that is co-released with insulin from pancreatic B-cells and the actions of this peptide on its target tissues maintains cell excitability and glucose hom
【机 构】
：
Luohe Medical College The First Affiliated Hospital of Luohe Medical College Hena China
【出 处】
：
BIT`s 3rd Annual World Congress of NeuroTalk-2012(2012第三届国际神
论文部分内容阅读
Regulated secretion of Human Amylin (hAmylin) that is co-released with insulin from pancreatic B-cells and the actions of this peptide on its target tissues maintains cell excitability and glucose homeostasis.Inappropriate control of hAmylin secretion results in human disease, in particular in Alzheimers disease.What receptors are acted by human amylin leads to neurotoxicity in neurons of brain is unknown.Nicotinic acetylcholine receptors (nAChRs) are known to play a critical role in a variety of nervous diseases.In the present study, we sought to determine the inter-relationships between these receptors by examining the actions of hAmylin and nicotine on whole-cell currents and membrane potential in basal forebrain neurons.Whole cell patch-clamp recordings were performed on enzymatically dissociated neurons of the diagonal band of Broca (DBB), a cholinergic basal forebrain nucleus.The results shown hAmylin or nicotine applied individually caused a dose-dependent (lnM-10μM) membrane depolarization and an increase in firing frequency of DBB neurons.Application ofAC253, an amylin receptor antagonist, blocked the excitatory effects of not only hAmylin but also nicotine, Dihydro-beta-erythroidine (DHβE), a non-7nAChR antagonist, blocked the effects of nicotine and also hAmylin.Similar effects were also observed in voltage-clamp mode.Bath application of hAmylin and nicotine resulted in reduction of whole cell currents in a voltage range between-30 and +30 mV.Nicotine and hAmylin depressed the potassium currents.DHBE attenuated the effects of hAmylin and nicotine.These electrophysiological results suggest that hAmylin receptor and nAChRs on DBB neurons are coupled and may function in a co-operative manner to influence the excitability of DBB neurons.These evidences are immensely important to understand the cause and mechanisms of Alzheimers disease.
其他文献
The immediate early gene, Arc (activity-regulated cytoskeletal-associated protein), regulates multiple forms of protein synthesis-dependent synaptic plasticity, including LTP, LTD, and homeostatic pla
Damage to peripheral nerves often results in various sensory and motor abnormalities.The loss of neurotrophic factor support has been proposed to contribute to the development of peripheral neuropathy
At neuromuscular synapses, agrin stabilizes postsynaptic acetylcholine receptor (AChR) clusters by signaling through the Muscle Specific Kinase (MuSK) complex.Imaging of cultured myotubes showed that
Brain plasticity refers brains ability to change according to physiological and pathological life events.Many different mechanisms participate to brain plasticity, including genesis of new neurons and
In Drosophila, Mushroom Bodies (MBs) are the site of CS-US association for olfactory memory.MBs receive olfactory CS inputs from cholinergic projection neurons and dopamine (DA) neurons convey the ave
Intent conditions influence human behavior, and the movement of the eye is used by humans to evaluate one anothers reasoning and detecting intent state.The purpose of this study was to investigate the
Values of global and regional cerebral blood flow, received in experimental and clinical investigation as the informative criteria for brain blood supply, are often misleading.Although it is known, th
Introduction: Bipolar disorder is a severe mental illness characterized by alternating bouts of mania and depression.The pharmacotherapy of bipolar disorder includes a variety of drugs, however, nearl
DNA topoisomerase Ⅱ (topo Ⅱ) cleaves both strands of a duplex DNA, allows another duplex to pass through the gap, and finally re-seals the gap.Analyses of developing rat cerebellum have revealed that
There is abundant evidence suggesting the relevance of glutamate to depression and antidepressant mechanisms.Curcumin, a major active compound of Curcuma longa, has been reported to have the biologica