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Nanomaterials usually will produce many unexpected results in the fields of bio-applications.Recently, our group developed several bio-applications of silicon nanowire arrays (SiNWAs) modified with thermo-responsive poly (N-isopropylacrylamide) (PNIPAAm-SiNWAs).The PNIPAAm-SiNWAs surface, previously reported to be native-protein resistant, is found to favor the adsorption of denatured proteins.The PNIPAAm-SiNWAs surface only absorbed small amounts of native proteins,whereas the amount of denatured proteins they absorbed was found to be notably higher.The amount of denatured protein absorbed also varied much more strongly with temperature.