【摘 要】
：
The complete coding sequence of one tobacco(Nicotiana tabacum)gene-ascorbate oxidase(AO),was amplified by RT-PCR.The complete coding sequence of tobacco AO gene was 1,722 bp which encodes a protein of
【机 构】
：
ongyun Honghe Tobacco(Group)Co.,Ltd.,Kunming,P.R.China
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The complete coding sequence of one tobacco(Nicotiana tabacum)gene-ascorbate oxidase(AO),was amplified by RT-PCR.The complete coding sequence of tobacco AO gene was 1,722 bp which encodes a protein of 573 amino acids.Sequence analysis revealed that the AO of tobacco shares high homology with the AO of Lycopersicon esculentum(89%),Populus trichocarpa(75%),soybean(74%),castor bean(73%)and peach(73%).The prediction of transmembrane helices showed that tobacco AO might be a transmembrane protein.The expression profile was studied and the results indicated that tobacco AO gene was differentially expressed in detected tobacco tissues including leaf,stem,root and flower.Our experiment established the foundation for further research on this tobacco gene.
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