【摘 要】
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Phosphatidylcholine (PC) is a kind of phospholipids,which has special characteristics and physiological function.It was widely utilized in the field of heal
【机 构】
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State Key Laboratory of food Science and Technology,School of food Science and Technology, Jiangnan
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Phosphatidylcholine (PC) is a kind of phospholipids,which has special characteristics and physiological function.It was widely utilized in the field of health product,cosmetic,food and pharmaceuticals industry.In particular,it is promising to prepare liposomes and pharmic emulsifier.A simple,rapid and accurate quantitative method was established for the determination of phosphatidylcholine by HPLC-UV in this research.Separation was obtained by using lichrospher Si (4.6×250mm,5 an) column and mobile phase of n-hexane/2-propanol (I∶I,V/V) and n-hexane/2-propanol/l% acetic acid (4∶4∶1) with flow rote of 1.0mL/min,etected wave was 205nm.Under this condition,PC and PE were well separated in 25min.Alcohol soluble phospholipids was detected contenting PC 40.08%(wt%) and PE 19.1%(wt%).In order to improve the PC content,a study has been started from alcohol soluble phospholipids with using macroporous ion-exchange resin S.
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