【摘 要】
：
The lower eukaryote Dictyostelium discoideum can exist as single-celled amoeba and as a multi-cellular organism and is a well-suited model for the investiga
【机 构】
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University of Cologne, Center for Biochemistry, Germany
【出 处】
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2005 World DNA and Genome Day(2005中国(大连)国际DNA和基因组节大会)
论文部分内容阅读
The lower eukaryote Dictyostelium discoideum can exist as single-celled amoeba and as a multi-cellular organism and is a well-suited model for the investigation of cell functions related to growth,motility,differentiation and development.To provide the genetic basis for future investigations the 34 Mb genome of this organism was sequenced,assembled and finished to a high level of accuracy.With a composition of 77% A+T,the genome could not be cloned in BACs as large insert clones,and was assembled using a chromosome by chromosome strategy,anchored through the production of a high-resolution STS map by the in vitro HAPPY map technique,and through the use of tiled congruent YACs.
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