Alfano introduced the potential use of optical fluorescence spectroscopy in 1984 and Raman spectroscopy in 1990 to diagnosis of human breast cancer and opened up the field of Optical Biopsy.Optical spectroscopy including emission,excitation, absorption, Stokes shift, synchronized diffusive reflectance and Raman, measures the allowed electronic transitions in photoactive bio-molecules and vibrational transitions from various functional groups in molecules.Fluorescence spectral scans of breast normal, malignant and adipose tissues were measured at CCNY on over eight hundreds ex-vivo specimens for the purpose of developing a spectroscopic diagnostic technique and compact devices.