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CopC functions by sequestrating and transferring copper with other Cop proteins in the oxidizing periplasmic space.The kinetics of copper(Ⅱ) transfer from CopC to MCopC,stimulation of Cop protein,in comparisons with to EDTA at pH 7.0 was investigated by fluorescence methods.The process of copper(Ⅱ) transfer from CopC to the chelator of EDTA in which the apparent rate constant for the decay process is linearly dependent on the EDTA concentration,is consistent with a mechanism and the rate law of directly exchanging ligants of copper(Ⅱ) by a single step.However,the copper(Ⅱ) transfer from CopC to MCopC is attributed to a two-step process consisting of a rapidly established pre-equilibrium (reaction 1) followed by a rate determining step (reaction 2),with a rate law described by eq 1.In addition,thermodynamics parameter and activation energy of copper(Ⅱ) transfer from CopC to MCopC were obtained.