[Objective] To screen out the optimal medium and explant for tissue cuhure of wild Lilium leuanthum.[Method]The tissue culture of wild Lilium leuanthum was studied using its scales, root pieces, stem pieces and leaves as explants respectively.The basic mediums were MS, Bζ and White.Four differential mediums were: MS+2.00 mg/L 6-BA+0.20mg/L NAA, MS+0.20mg/L 6-BA+1.00 mg/L IAA, MS+0.10 mg/L KT+0.10 mg/LNAA and MS+0.03 mg/L NAA.Six proliferation formulas were as below: MS+0.03mg/L NAA, MS+2.00 mg/L 6-BA+0.20mg/L NAA, MS+0.20 mg/L 6-BA+1.00 mg/L IAA,MS+0.10mg/L KT, MS+020 mg/L KT+0.20 mg/L NAA and MS+0.10 mg/L KT+0.15 mg/L NAA.Four rooting media were: MS+1.00 mg/L IBA, MS+1.00 mg/L IBA+0.20 mg/L 6-BA, 1/2MS+1.00 mg/L IBA+0.20 mg/L 6-BA and White+1.00 mg/L IBA+0.20mg/L 6-BA.[Result] MS medium was the optimum basic medium.The optimal differential mediums for scale of wild Lilium leuanthum was MS +0.10 mg/L KT+0.10 mg/L NAA, for root pieces and stem pieces of wild Liliumn leuanthum were MS+ 2.00 mg/L 0-BA +0.20 mg/L NAA, for leaf pieces was MS +0.10 mg/L KT+0.10 mg/L NAA, respectively.The optimum subculture medium was MS+ 0.10 mg/L KT +0.15 mg/L NAA and the optimal medium for rooting of rootless bulbs and seedlings was 1/2MS+0.20 mg/L 6-BA+1.00 mg/L IBA.In the field, the tissue culture seedlings formed by scales and roots grew more strongly than others.[Conclusion] The optimal tissue culture mediums and explants for wild Lilium leuanthum were obtained in this work, which provided basis for the rapid propagation of wild Lilium leuanthum.