Construction of a Specific MFC-based Biosensor for Copper by Employing Shewanella piezotolerans WP3

来源 :第二十次全国环境微生物学学术研讨会 | 被引量 : 0次 | 上传用户:yu19910108
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  Environmental pollution by heavy metals is a serious problem around the world.However,Traditional electrochemical devices are expensive,inaccurate,or cumbersome for practical applications.MFC based biosensor has shown a potential as an effective and economical method for determining the quantity of hazardous metals.In this study,we designed an engineering ShewanellapiezotoleransWP3 and applied it in MFC based biosensor for specific copper detection.Bacteria have been demonstrated that can utilize negative regulator system to sense the variation of intracellular heavy metals concentration and control structure gene expression to maintain homeostasis.CopA promotor and its repressor expressing geneCueRwere selected and fused into PSW4 to regulate downstreamribB expression,which encoded for the riboflavin biosynthetic enzyme 3,4-dihydroxy-2-butanone-4-phosphatesynthase.Expressive discrepancy of riboflavin under control of divergent copper concentration,as an electron shutter,can be reflected into the voltage variation in MFC.
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