【摘 要】
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We reported the development of two- and three-input logic devices based on the fact that Hg2+-,Ag+-,and/or coralyne can drive fluorescence quenching of oligonucleotide-based probe.The designed probe i
【机 构】
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Department of Chemistry,National Sun Yat-sen University,Kaohsiung,Taiwan(R.O.C)
【出 处】
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第八届全国微全分析系统学术会议、第三届全国微纳尺度生物分离分析学术会议暨第五届国际微化学与微系统学术会议
论文部分内容阅读
We reported the development of two- and three-input logic devices based on the fact that Hg2+-,Ag+-,and/or coralyne can drive fluorescence quenching of oligonucleotide-based probe.The designed probe is a single-stranded oligonucleotide that contains a a carboxyfluorescein (FAM) at the 5-end and a 4-([4-(dimethylamino)phenoyl]azo)-benzoic acid (DABCYL) at the 3-end.Becuase a single-stranded oligonucleotide consists of a series of 58 random DNA bases (DNA58,GTC TCT GTG TGC GCC AGA GAA CAC TGG GGC AGA TAT GGG CCA GCA CAG AAT GAG GCC C),Hg2+-,Ag+-,and coralyne can force the conformation change of the DNA58 from a random coil to a folded structure via the coordination of thymine-Hg2+- thymine,cytosine-Ag+-cytosine,and adenosine2-coralyne-adenosine2,respectively.This conformational change induced direct contact between FAM and DABCYL,causing collisional quenching of fluorescence.The addition of thiol-containing molecules and chelating agents to a solution of the Hg2+-DNA58 complexes resulted in the restoration of the fluorescence of FAM.Similar fluorescence response was observed in the case of the Ag+-DNA58 complexes.Moreover,the fluorescence of the coralyne-DNA58 complexes lighted up in the presence of heparin.According to the abovementioned results,a set of logic gates involving two-input and three-input systems was demonstrated,including OR,AND,NOR,INH,IMPLICATION and three-input NOR gate.These results displays the higher order circuts for logic gate operation based on oligonucleotide-based probe.
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