【摘 要】
：
Trace metabolites,such as modified nucleosides,neurotransmitter and steroid hormone,play important roles in organism.However,their determination from comple
【机 构】
：
KeyLaboratoryofSeparationScienceforAnalyticalChemistry,DalianInstituteofChemicalPhysics,ChineseAcade
【出 处】
：
2016年分析化学前沿国际研讨会及中美分析化学研讨会
论文部分内容阅读
Trace metabolites,such as modified nucleosides,neurotransmitter and steroid hormone,play important roles in organism.However,their determination from complex biological samples is still a challenging task due to their trace content,strong polarity and low ionization efficiency etc.In this study,we developed a set of strategy to solve these issues.Firstly,novel nano/micro materials were synthesized for selectively removing the matrix interference and enriching the targets from complex samples.Secondly,we developed novel stable isotope tagged derivatization reagents and chemoselective nanoprobes to further improve the sensitivity and retention of trace metabolites.Thirdly,the development of novel analytical columns are also important for separation of polar trace metabolites.A set of HILIC stationary phases confined zwitterionic IL,glucaminium IL,dicationic IL and tricationic IL were prepared with high column efficiency and resolution.Finally,we developed a novel nontargeted modification-specific metabolomics method based on ISCID LC-HRMS for large-scale detection of trace modified metabolites.In conclusion,the determination of trace metabolites from biological samples will be gradually overcome with the development of new materials for sample pretreatment and separation.
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