【摘 要】
：
The transcription factor Spl is ubiquitously expressed in different cells and thereby regulates the expression of genes involved in many cellular processes.This study reveals that Spl was phosphorylat
【机 构】
：
Institute of Signal Transduction National Cheng Kung University Taiwan
【出 处】
：
BIT Life Sciences 1st Annual World Cancer Congess-2008(2008中
论文部分内容阅读
The transcription factor Spl is ubiquitously expressed in different cells and thereby regulates the expression of genes involved in many cellular processes.This study reveals that Spl was phosphorylated during the mitotic stage in three epithelial tumor cell lines and one glioma cell line.By using different kinase inhibitors, we found that during mitosis in HeLa cells the c-Jun N-terminal kinase 1 (JNK1) was activated that was then required for the phosphorylation of Spl.In addition, blockade of the Sp 1 phosphorylation via an inhibition of the JNK1 activity in mitosis resulted in the ubiquitination and degradation of Spl.JNK1 phosphorylated Sp 1 at threonine 278/739.The Sp 1 mutated at threonine 278/739 was unstable during mitosis, possessing less transcriptional activity for the 12(S)-lipoxygenase expression, and exhibiting a decreased cell growth rate when compared to wild-type Sp 1 in HeLa cells.
其他文献
Background and aims: Recent findings suggest that growth factor receptor-mediated motility is one of the most common aberrations of tumour cells that render them invasive.The epidermal growth factor (
The identification of novel tumor suppressors altered in breast cancer is an important issue for tumor classification, prognosis and treatment.Frequent allelic deletions on the short arm of human chro
Purpose: The Wnt genes are involved in embryogenesis and tumorigenesis.Among them, Wnt1 stimulates the canonical Wnt/beta-catenin signaling pathway, which regulates the transcription of many Wnt-targe
Accurate HPV genotyping is important for clinical follow-up of persistent HPV infection, a recognized tumor promoter in cervical cancer induction, and for evaluation of prevention strategy for the ind
To evaluate whether HPV genotyping for the selected oncogenic HPV types can improve the efficacy ofHPV DNA testing in predicting CIN2/3 in women with the mildly abnormal Pap results, we compared HPV D
Intrahepatic expression of hepatitis B x antigen (HBxAg) is associated with the development of HCC, perhaps through up-regulated expression of selected cellular genes.Accordingly, HepG2 cells were sta
Widely spread sexually-transmitted diseases like genital herpes, caused predominantly by the infection with Herpes simplex virus (HSV) type 2, and human papilloma virus (HPV) infections pose considera
Recent studies have demonstrated genetic alterations in the normal epithelial and mesenchymal tissues surrounding breast carcinomas.These data strongly suggest that genetic abnormalities in microenvir
Tobacco-smoking is one of the well understanding carcinogenic factors involved in breast cancer formation.In this study, we first demonstrated that the several types of nicotinic acetylcholine recepto
The receptor for atrial natriuretic peptide (ANP), natriuretic peptide receptor A (NPRA), is expressed in cancer cells, and natriuretic peptides have been implicated in cancers.However, the direct rol