【摘 要】
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OBJECTIVE:To assess the effect and dose-response relationships of Tanreqing injection on Lipopolysacchaide(LPS)induced airway mucus hypersecretion in HBE16 airway cells.METHODS:To explore the inflamma
【机 构】
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Department of Integrated Traditional Chinese and Western Medicine,West China Hospital, Sichuan Unive
【出 处】
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中国医师协会中西医结合医师分会呼吸病专家委员会第二届学术研讨会
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OBJECTIVE:To assess the effect and dose-response relationships of Tanreqing injection on Lipopolysacchaide(LPS)induced airway mucus hypersecretion in HBE16 airway cells.METHODS:To explore the inflammation model of HBE16 cells by LPS with optimal processing time and concentration by CCK-8 method;to explore the maximum concentration of TRQ and baicalin for HBE16 cells by CCK-8 method;according to the maximum concentration of TRQ and baicalin for HBE16 cells,the groups were divided into control group,LPS group,LPS plus drug intervention group(TRQ:1:32,1:64,1:128,1:256,1:512;baicalin:10 μg/ml、50 μg/ml、100 μg/ml);the secretions of MUC5AC of HBE16 cells treated with LPS plus TRQ or baicalin in cell culture supernatants were quantified by enzyme-linked immunosorbent assay(ELISA).Tthe messenger RNA(mRNA)expressions of MUC5AC of HBE16 cells treated with LPS plus TRQ or baicalin in cell RNA were tested by quantitative real-time polymerase chain reaction(real-time RT-PCR).The expression of phospho-IKKα/β,phospho-NF-κB p65 and phospho-IκB-α of HBE16 cells treated with LPS plus TRQ or baicalin for 24 hours were tested by Western blotting to determine whether the signaling pathway NF-κB was involved in the anti-inflammatory action of TRQ and baicalin.
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