Purpose: Studies indicated mesenchymal stem cells(MSCs),derived from bone marrow or adipose tissue etc.could enhance diabetic wound healing in rats.However,clinical applications in wound healing are still concerned under FDA situation.We investigated whether cultured medium of adipose-derived stem cells(ASCs)under hypoxia-precondition could enhance diabetic wound healing in a STZ-induced diabetic rodent mode Materials and methods: Rat diabetes would be induced in male Wistar rats(weigh: 300-350 g)by a single injection of streptozotocin(STZ)(65 mg/kg i.p.).Rat dorsum was shaved and a 6×5-cm dorsal skin was drawn on the rats as animal model followed our previous report.Diabetic rats received different dosage of hypoxia conditioned medium in wounding bed post-operatively.The expression levels of the growth factors and related-cytokines were evaluated.Results: The results revealed the morphological changes of ASCs did not show obvious difference between human and rat ASCs under hypoxia and normoxia conditioning.VEGF and related cytokine were significant increased in hypoxia group,as compared to that in normoxia.HIF-1 expression of nuclear extracts of rat ASCs revealed significant increase.Our in vivo animal study showed hypoxia-induced ASC-conditioned culture medium could enhance diabetic wound healing as compared to that in controls in a STZ–induced diabetes rodent dorsal wounding model.Conclusion: Hypoxia-induced ASC-conditioned culture medium could enhance diabetic wound healing and should not one option for critical wound healing.