【摘 要】
：
To create germplasm and detect the genetic basis of heat tolerance in rice,we developed a set of contiguous segment substitution lines(CSSLs)through repeated backcrossing and molecular marker assistan
【机 构】
：
School of Agronomy of Anhui Agricultural University.Hefei,230036,China
论文部分内容阅读
To create germplasm and detect the genetic basis of heat tolerance in rice,we developed a set of contiguous segment substitution lines(CSSLs)through repeated backcrossing and molecular marker assistant selection(MAS).These CSSLs were derived from a cross between N22 as donor,with special heat tolerance,and 9311 as recurrent parent,which is susceptible to high temperature but known as an elite restore line of super-hybrid rice in China.The advanced backcross generations(BC3 and BC4)were subjected to three rounds of MAS to select lines with single or few introgression chromosomal segments of N22.One hundred and forty one polymorphic simple sequence repeat(SSR)markers evenly distributed on rice genome were used in the MAS scheme.A set of 113 CSSLs was determined by graphical genotypes,each of lines contained a single or few chromosomal introgression segments with the background of 9311,all the overlapped segments covered the whole genome of N22.The genome proportion of the recurrent parent in each of the CSSLs ranged from 92.1％ to 98.2％,with an average of 96.2％.The average proportion of homozygous and heterozygous donor segments were 1.3％ to 0.5％,respectively.The average length of introgression segments was 21.5cM,ranged from 0.5cM to 45.2 cM.These CSSLs will be useful in identifying the QTLs for many favorable traits,especially for heat tolerance,and detecting the action and interaction of the QTLs.
其他文献
Arbuscular mycorrhizal(AM)is a mutualistic symbiosis wildly formed between AM fungi and the roots of terrestrial plants.Citrus have rare or short root hairs and thus are fairly depends on AM symbiosis
Proanthocyanidins were qualitatively and quantitatively identified using hydrolysis and thiolysis assays,NP-HPLC,HPLC-ESI-MS,MALDI-TOF-MS,1H-NMR,and 13C-NMR techniques in different organs of tea plant
Soybean is one of the most important crops,providing large amounts of dietary proteins and edible oil,and is also an excellent model for studying evolution of duplicated genes.However,relative to the
Accumulating evidence show that sulfur dioxide participates in various biological processes in animals,but there is limited information on the regulatory role of SO2/sulfite in plants.Here we show tha
Regulation of gene expression is associated with RNA interference(RNAi)at the post-transcriptional level and chromatin modification in transcriptional silencing during plant development.Dicer-like(DCL
Lipoxygenases(LOXs)are important dioxygenases in cellular organisms and contribute to plant developmental processes and environmental responses.Although the genome-wide analysis of LOXs has been condu
Boron(B)is an essential micronutrient for plant growth and development.While Oilseed rape(Brassica napus)needs much B and showedmore sensitive to low soil boron availability.Nowadays,the emergence of
14-3-3 proteins are a group of regulatory proteins of divergent functions in plants.However,their functionalities have hardly been known in maize kernel development,a process of pivotal significance t
Phyllotaxy pattern development is regulated by integrated networks of gene expression,hormonal signaling,and metabolite sensing.In this study,we characterized differentially expressed genes(DEGs)betwe
MAPK cascades have been implicated in various developmental processes and in response to stresses in plants.In this study,we cloned and characterized a Group A MAPKK gene,designated BdMKK6.2,from Brac