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This abstract discusses the application of XRF technology for the analysis of major and minor element in biological samples.Microbeam X-ray fluorescence (micro-XRF) spectrometry has been raised as an analytical technique of microbeam during the recent years.With its advantages of high sensitivity, small sample requirement, high testing accuracy and non-destruction, the technique is widely utilized in biological samples.Various synchrotron-based techniques are currently available that permit such investigations in situ and at the molecular level.The continuous development of these techniques is delivering substantial gains in terms of sensitivity and spatial resolution, which allows analyses of diluted samples at the submicron scale.