以往研究表明 ,外根鞘的隆突区可能存在毛囊干细胞[1] ,利用干细胞的无限增殖能力和多能分化特性可以修复创面 ,提示外根鞘细胞在创面愈合领域具有重要的研究价值。笔者利用鼠尾胶原作底物 ,分别采用组织法和胰蛋白酶消化法进行了外根鞘细胞的培养 ,获得成功。材　料　与　方　 Previous studies have shown that there may be hair follicle stem cells in the carina of the outer root sheath [1]. It is possible to repair wounds using the unlimited proliferative capacity and pluripotency of stem cells, which suggests that the ECM plays an important role in wound healing. The author used rat tail collagen as a substrate, respectively, by tissue method and trypsin digestion method of outer root sheath cell culture, to be successful. Material and side