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Background and objective A previous study demonstrated that miR-126 can inhibit colon cancer proliferation and invasion by its negative regulationi of CXCR4 and RhoA signaling pathway.This study was to investigate the relationship between miR-126 and CXCR4/RhoA signaling pathway and explore whether CXCR4 plays key role in the process of miR-126 targeting RhoA pathway.