Ionic liquids (ILs) are entirely composed of cations and anions,which have unique properties such as environmental benefits,nonvolatility,noninflammability,high thermal stability and designability.In recent years,it seemed to ILs in high-speed counter-current chromatography (HSCCC) has attracted scientists’ attention.However,to date,there are no reports that discussed the mechanism about ILs applied to HSCCC.