Objective:To explore the distribution and antibiotics resistance of non-fermenting bacteria in intensive care unit to guide rational application of antibiotics in clinic.Methods: The non-fermenting bacteria isolated from sputum specimens of patients in intensive care unit from 2008 to 2009 were identified and their drug resistance were analyzed.Results: A total of 4273 strains of non-fermentating bacteria were isolated, representing an isolation rate of 38 %, The dominant 4 strains were Pseudomonas aeruginosa(44.09%), Acinetobacter baumannii(27.64 %), Stenotrophomonas maltophilia(10.58%) and Burkholderia cepacia(5.99%).High drug resistance to common antibiotics, excluding fromβ-lactamase inhibitor compound for instance cefoperazone/sulbactam, was revealed in the four species of non-fermenting bacteria.