The First Ratiometric Fluorescent Probe for Aminopeptidase N

来源 :第九届IUPAC化学生物学国际研讨会暨第八届世界华人药物化学研讨会 | 被引量 : 0次 | 上传用户：adamsqiu

【摘要】

：

The current work describes the design and synthesis of the first ratiometric fluorescent probe,Ala-PABA-7HC,for the detection of aminopeptidase N(APN) in both enzymatic and cellular level.Upon adding

【作者】

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Laizhong Chen Wei Sun Wenhua Li Jing Li Lupei Du Wenfang Xu Fang Hao Minyong Li

【机构】

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Department of Medicinal Chemistry,School of Pharmacy,Shandong University,Jinan,Shandong 250012

【出处】

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第九届IUPAC化学生物学国际研讨会暨第八届世界华人药物化学研讨会

【发表日期】

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2012年8期

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　　The current work describes the design and synthesis of the first ratiometric fluorescent probe,Ala-PABA-7HC,for the detection of aminopeptidase N(APN) in both enzymatic and cellular level.Upon adding APN to the solution of Ala-PABA-7HC in PBS or culture medium,the maximum emission peak experienced a red shift from 390 nm to 450 nm,as well as the ratio of fluorescence intensity(I450/I390)(λex=330nm) significantly increased from 0.26 to 3.05(R=11.7-fold) in 15 min.

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The First Ratiometric Fluorescent Probe for Aminopeptidase N

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