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Rotted yellow tissue taken from Pleurotus eryngii fruit bodies grown at an industrial mushroom cultivation facility was used to create a bacterial 16S rDNA library.Random clones were screened and obtained using the restriction endonucleases MspⅠ and RsaⅠ, and the 16S rDNA sequences of representative clones were identified.A BLAST search revealed the presence of two bacterial types in the diseased tissue, which exhibited 97% and 93% similarity with Pseudomonas and Bacillus, respectively.