【摘 要】
：
In order to get higher extraction efficiency,the polysaccharides from Sparassis crispa were extracted using cellulase.A single-factor study was designed to investigate the effects of different factors
【机 构】
：
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
论文部分内容阅读
In order to get higher extraction efficiency,the polysaccharides from Sparassis crispa were extracted using cellulase.A single-factor study was designed to investigate the effects of different factors on the yield of polysarccharides,such as enzymatic addition,enzymatic hydrolysis temperature,enzymatic hydrolysis time,liquid/material ratio and pH value.Based on the results of single-factor assay,several factors were chosen to be further optimized by response surface methodology.The optimal process parameters are shown as following: pH 5.5,extraction temperature of 40 ℃,enzymatic addition of 1.3 %,hydrolysis time of 2.5 hours,and the material/liquid ratio of 42 mL/g.Under these above conditions,the content of polysaccharides could be up to (22.90 %±0.26 %).The influence of different type macropore resins on decolorization rate and polysaccharide remaining rate of crude polysaccharides extracted on specific conditions,the combination of macropore adsorption resins and macropore anion exchanger got the best results(decoloration rate 80.31% and polysaccharide remaining rate 83.31%).Then with DEAE-52 cellulose was applied to purificate polysaccharide by column chromatography method.The yield of neutral polysaccharides and acid polysaccharides were 7%and 0.9%,respectively.
其他文献
[Objective]The aim of this work was to study the molecular machanism of SigB in biofilm formation by Listeria monocytogenes.[Methods]Here,Listeria monocytogenes WaX12 and WaX12-△sigB biofilm was inves
Mulberry (Morus alba L.) was considered as delicious and nutritious fruit,but difficult to storage,which was an obstacle for selling and further processing.The objective of this study was to explore c
[Objective]The study aimed to further explore the immunomodulatory properties of Polysaccharides from Dendrobium Officinale Leaves.[Methods]Column chromatography was used to separate and purificate po
Grass carp as raw materials,electronic nose and solid phase micro-extraction (SPME)combined with gas chromatography-mass spectrometry(GC-MS) to study the way of death effects on the volatile component
The inhibition effects of lysozyme,sodium acetate trihydrate and glycine alone on Listeria monocytogenes by measuring the antibacterial rate,The responses surface methodology(RSM) was utilized to opti
The incidence of obesity and overweight has increased dramatically recently,now constituting a serious threat to public health.Currently,functional food and food ingredient are attracting more and mor
To research the effect of vibration on Hami melons cell wall during storage,to research the effect of different grade road vibration on Hami melons storage quality during the transport process,simulat
[Objective]Screening and Identification of the Primary Bacterium from the WIC preparation.[Methods]It is used the method of pure culture,Selected 6 main strains,which were obvious difference in the sh
[Objective]To study the effect of edible mayonnaise on the absorption rate of calcium in rats.[Methods]Choose 6 weeks of age weight there was no significant difference in SD rats (n =20) and were divi