Background.Bladder cancer is a common malignant tumor with a very high recurrence rate after surgery.Intravesical instillation can help clear up the residual tumor cells after surgery and thereby reduce the recurrence rate.Objective.To establish a bladder tumor transplantation animal model and to evaluate the inhibitory effects of a novel perfusate, Su Funing Lotion (SFN), on bladder tumor.Methods.SFN was compared with several commonly used chemotherapy drugs, including mitomycin (MMC) and pirarubicin (THP) for anticancer effects on the bladder cancer cell lines T24, BTT, and BIU-87 and SFN half inhibitory concentrations (ICS0) were determined after 48 hours of treatment.In addition, bladder cancer orthotopic transplantation tumor models were established in BALB/C nude mice and T739 mice, and SFN anticancer effects were assessed in vivo, with normal saline and MMC as negative and positive controls, respectively.Results.SFN, MMC, and THP were all lethal to bladder cancer cells, in vitro, with SFN and THP significantly superior to MMC.IC50 values for SFN were 13.22, 11.22, and 12.5 μg/mL on T24, BTT, and BIU-87 cells,respectively.In vivo, SFN significantly reduced the mouse bladder wet weight and prolonged the animal survival compared with controls (P <.05), suggesting that SFN significantly inhibited T24/BTT cell growth in mice.Conclusion.SFN inhibited the bladder cancer cell proliferation in vitro and in vivo and significantly prolonged the survival of mice with bladder cancer xenografts, indicating that SFN could be used as a perfusate after surgery for removal of residual bladder cancers cells.