【摘 要】
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Objective: To study the pharmacokinetic of SM-1,a newly synthesized antitumor drug,in rat plasma.Methods: A LC-MS/MS method was developed and validated for the determination of SM-1 in rat plasma.SM-1
【机 构】
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Haikou Hospital of Xiangya Medical College , Central South University Haikou 570208 , Hainan Provinc
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Objective: To study the pharmacokinetic of SM-1,a newly synthesized antitumor drug,in rat plasma.Methods: A LC-MS/MS method was developed and validated for the determination of SM-1 in rat plasma.SM-1 and internal standard were separated with gradient elution using acetonitrile-methanol-10raM ammonium acetate (35.5∶35.5∶25,v/v/v)as mobile phase at a flow rate of 0.6 ml/min.The analytes were monitored by electrospray positive ionization with multiple reaction monitoring mode.Results: All validation parameter results were within the acceptable range described in guideline for bioanalytical method validation.The method was successfully applied to pharmacokinetic study of SM-1 in rats.Conclusions: This research provides a scientific basis and reference value for the further research of SM-1.
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