Objectives (1) To obtain pure and primary cultured oligodendtocyte/type-2 astrocyte (O2A) progenitors from cortices of neonatal rats.(2) To determine the expression and function of FP 1 on the primary cultured O2A progenitors.(3) To establish the feasible hypoxic-ischemic PVL model in vitro.(4) To ascertain the expression and effect on the PVL model in vitro.Methods (1) The O2A progenitors were isolated and primarily cultured from the cortices of neonatal SD rats by twice shaking and using the conditioned medium.The morphologies of O2A were observed under inverted phase-contrast microscope.And the immunofiuorescence staining was used to identify and determine the purity of the ceils.(2) Double-label immunofluorescence assay was used to investigate the expression of FP1 on the O2A progenitors and iron efflux test was used to explore the function of FP1.(3) O2A progenitors were treated with Oxygen-glucose deprivation (OGD) for 3, 6, 12, 24 h to mimic hypoxic-ischemic PVL in vitro and their morphologies were observed under inverted phase-contrast microscope.The survival rate of O2A progenitors was investigated by CCK-8 method and testing the lactate dehydrogenase (LDH) leakage.(4) The level of FP 1 in the PVL model in vitro was tested by reaI time PCR and Western blot.Results (1) O2A progenitors were round or oval with strong refraction.They had monopolar, dipolar or tripolar processes, which were fine and straight with no branches, O2A progenitors were A2B5 positive cells and their purity is up to 95%.(2) Double-label immunofluorescence assay showed that O2A progenitors can express FP1, which was located in the cell membrane, body and processes.(3) Iron efflux test showed that the immunofluorescence intensity increased with time-dependence in the routine cultured and OX42 antibody treated O2A progenitors (P>0.05).While the immunofluorescence intensity of FP1 antibody treated cells did not increase with time.(4) After OGD 3 h, 6 h, 12 h and 24 h, the morphologies of O2A progenitors had hypoxic-ischemic changes, and the survival rate decreased and the LDH viability in the cultured medium increased with time-dependence.(5) The level of FPlmRNA and protein decreased with time-dependence after OGD 3 h, 6 h and 12 h.Conclusions (1) O2A progenitors with purity up to 95% can be obtained by twice shaking and using conditioned medium.(2) O2A progenitors can express FP1, which was located in the cell membrane, body and processes.(3) FP1 can induce the iron efflux from O2A progenitors and it is an important iron-regulated protein.(4) The feasible hypoxic-ischemic PVL model in vitro can be successfully established by treating the O2A progenitors with OGD3 h, 6 h and 12 h.(5) The level of FP1 in the hypoxic-ischemic PVL model in vitro decreased with time-dependence, which may reduce the iron efflux from the cells and then aggravate the cell hypoxic-ischemic injury.