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To evaluate the repair effect of Morida Officinalis Polysaccharide (MOP) on the Sertoli cell tight junction(TJ) proteins(occluding, JAM-A,claudin-11,ZO-1),androgen receptor (AR), testosterone(T) and cytokines(TGF-β3,TNF-α,IL-6)of experimental varicocele in Rats,75 adolescent male SD rats were randomly divided into sham,model,model+drug 200mg/kg, model+drug 300mg/kg and model+drug 400mg/kg groups.A varicocele rat model was established by partial ligation of the left kidney vein.Eight weeks after operation, the testis tissues in different groups were analyzed by immunofluorescence, immunohistochemistry, transmission elctron microscopy, Weatern blot,ELISA and qPCR.The results showed that varicocele down-regulated the expression TJ proteins (occluding, claudin-11,ZO-1)and AR;didn't change the expression of JMA-A;increased the level of cytokines and decrease the level of T in left testis.Meanwhile, MOP up-regulated the expression of TJ proteins (occluding,claudin-11,ZO-1)and AR,decreased the level of cytokines and T.We conclude that MOP can repair the damaged TJ in rats caused by experimental varicocele via decreasing the level of cytokines (TGF-β3,TNF-α,IL-6) and up-regulating the expression of TJ proteins (occluding,claudin-11,ZO-1) and AR.