【摘 要】
：
Yangjing Capsule (YC) extract,a traditional Chinese medicine compound,has been shown to increased testosterone production and the expression of StAR,CYP11A1,and HSD3B mRNAs and proteins.However,the mo
【机 构】
：
Institute of Andrology,Nanjing University of Chinese Medicine,Nanjing,Jiangsu 210023,China
【出 处】
：
中华中医药学会第十四次男科学术大会
论文部分内容阅读
Yangjing Capsule (YC) extract,a traditional Chinese medicine compound,has been shown to increased testosterone production and the expression of StAR,CYP11A1,and HSD3B mRNAs and proteins.However,the molecular events behind the enhancement of testicular steroidogenesis are unknown.The nuclear receptor NUR77 is present in testicular Leydig cells,and its expression induce promoter activity of steroidogenic enzyme genes including StAR,P450c17,and HSD3B.In this study,Real-time PCR and western blot were applied to determine the mRNA and protein expression of NUR77.NUR77 knockdown were applied to explore the underlying mechanism.Transient transfections in MLTC-1 cells were applied to confirmed the effect on NUR77 promoter activity.The Results showed that YC extract significantly stimulated NUR77 production in dose-and time-dependent manners.Ad-siRNA-NUR77 treatments held back YC extracts stimulation effects on mRNA and protein expression of StAR and HSD3B and consequently inhibited the testosterone synthesis of MLTC-1 induced by YC extract.YC extract can activate the NUR77 promoter.All together,our results implicate NUR77 as a mediator of YC extract action on steroidogenic enzyme transcription in steroidogenic Leydig cells in this process.
其他文献
所谓“痰瘀互结，毒损宗筋”的思维，首重宗筋的功能改善，摒弃了惯有的思路，通过荣养宗筋而纠正宗筋弛纵治疗阳痿，能有效地改善阴茎勃起功能，是中医学治疗阳痿惯有思路的新拓展，是在代谢类疾病逐年高发的情况下，对本病新的探索和思考，通过对临证经验的凝练，升华了学术思想，形成新的治疗思维和法则。
为了继承和发扬孙建明教授治疗早泄经验,通过长期跟师名中医,总结孙建明教授治疗早泄经验,包括以肝肾同源为理论基础,注重补益肝肾;同时注重以水火既济为理论基础,主张从心论治;此外,以五运六气立论,根据司天、在泉、主运、主气、客气等的不同,分别加用针对不同气运的药物.
慢性前列腺炎对男性生育的影响是复杂的。主要表现在对男性精液质量，包括精子密度、活力等。由于精浆中前列腺液为炎性改变，使精液不液化，或液化时间延长。前列腺是雄激素非常敏感的组织。如果前列腺组织炎性改变，在先天性5a—还原酶缺乏的情况下，使睾酮向双氢睾酮的转化受到了影响，编码硫酶的基因有29种变异。睾丸内虽有精子产生，但却不能生育。
目的：观察桃红前列汤联合前列解毒胶囊治疗慢性前列腺炎的临床疗效.方法：将120例患者按随机数字表法分为对照组和观察组，每组各60例，对照组给予前列解毒胶囊治疗，治疗组在此基础上加用桃红前列汤治疗，治疗8周后比较两组临床疗效.结果：治疗组总有效率为88.5％，对照组总有效率为81.1％，两组比较差异具有统计学意义(P＜0.05)；治疗组治疗后WBC计数、N1H-CPSI评分和中医证候评分分别为9.4
目的：观察中药自拟方金龙汤灌肠对湿热瘀滞型慢性前列腺炎的临床疗效.方法：将68例慢性前列腺炎患者随机分为治疗组(金龙汤灌肠组)和对照组(前列安栓组)，每组有效病例34例.治疗14天后对比观察两组的评分变化(NIH-CPSI评分+中医症候评分).结果：两组评分统计前、后具有显著差异性(P＜0.05)，且组间比较也具有差异性，治疗前后肝肾功比较(P＞0.05)，均未发现明显毒副作用.结论：自拟方金龙汤
目的：观察中西医结合治疗Ⅲ B型慢性前列腺炎的临床疗效.方法：符合Ⅲ B型慢性前列腺炎患者120例，采用随机对照，分为治疗组和对照组两组，治疗组(60例)用盐酸坦索罗辛缓释片和前列安通片，对照组(60例)口服盐酸坦洛新缓释片，两组均治疗4周，观察比较临床疗效.结果：治疗组治愈19例、显效22例、有效14例、无效5例，总有效率91.7％；对照组治愈6例、显效16例、有效18例、无效20例，总有效率6
目的：通过观察龙附颗粒对慢性前列腺炎(chronic abacterial prostatitis,CAP)模型大鼠前列腺组织细胞因子白细胞介素-10 (IL-10)及肿瘤坏死因子-α (TNF-α)表达的影响,探讨其可能的作用机制.方法：将48只wistar大鼠随机分为正常对照组、CAP模型组、舍尼通组、龙附颗粒低、中、高剂量组,每组8只.通过HE染色法观察各组大鼠前列腺组织形态学的差异,运用E
目的：观察补肾活血通淋方对良性前列腺增生症大鼠模型Bax、Bcl-2及其比值的影响.方法：72只Wistar大鼠被随机分为6组，即假手术组、模型组、保列治组、补肾活血通淋方低、中、高剂量组.除假手术组外，余组采用摘除大鼠双侧睾丸后连续注射丙酸睾丸酮30天的方法建立模型，在造模的同时，给药组每天灌胃给药1次，连续30天.采用RT-PCR法测定BaxmRNA和Bcl-2mRNA的表达强度并计算其比值.
Objective: To observe the protective effects of Yangjing capsule on testicular damage of adult male mice induced by cyclophosphamide(CP) and the mechanism.METHODS: Fifty adult male mice were randomly
Objective: To investigate the role of Pou3f1 in proliferation of GC-1 spermatogonia (spg) cells cultured by Yangjing Capsule (YC) extract.Methods: GC-1 spg cells were treated with 0.01,0.1,and 1 mg/mL