【摘 要】
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Objective:Microarray for gene expression profiles was used to screen out the differentially expressed genes after cardiopulmonary bypass in peripheral blood
【机 构】
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Department of Cardiopulmonary bypass,Cardiovascular Institute & Fuwai Hospital,PUMC and CAMS,Beijing
【出 处】
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第七届亚洲体外循环学术会议暨第三届全国体外循环学术会议
论文部分内容阅读
Objective:Microarray for gene expression profiles was used to screen out the differentially expressed genes after cardiopulmonary bypass in peripheral blood mononuclear cell(PBMC) to determine the application values of gene chip technique in cardiac surgery with cardiopulmonary bypass (CPB).The candidate genes and its functions were primarily analyzed and forecasted.Methods:20 patients underwent open heart surgery under CPB were selected.The patients artery bloods were drown before and after CPB.PBMC were obtained from heparinized blood by Ficoll gradient centrifugation.
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