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Organic fluorescent dyes such as Cy3,Cy5 and Alexa Fluor 647 are widely used as fluorophores in single molecule localization microscopy(SMLM)[1,2].Based on the imaging principle of SMLM,the photophysical properties of fluorophores,e.g.the photostability,fluorescence lifetime and quantum yields,play an important role in the localization precision and spatial resolution [3,4].