Continuous interest has been put on developing all-solid-state ion-selective electrodes(ISEs)with lower detection limit,higher potential stability and reproducibility and longer life time.Different materials have been used as solid transducer such as Ag|AgCl,redox-active self-assembled monolayer,Prussian blue,carbon-based materials and conducting polymers(CPs).However,most CPs are photosensitive and partially hydrophilic making them not ideal transducer materials.Other materials also have their own shortcomings,such as low redox capacitance of electroactive monolayers,and hydrophility of carbon-based materials.MPCs as solid contact transducer material can provide a clearly-thermo-defined,electrochemical-reversible,non-polarizable interface.In addition,MPCs are more hydrophobic than other materials,which can effectively eliminate formation of water layer.The traditional method to synthesize MPCs was by a two-phase method at 0 ℃ requiring a rather low stirring rate.The synthesis time was long with a low yield(20-30%).The relatively complicated synthetic procedure and the short lifetime of the resulting ISE restricted the mass production and practical applications of those devices.Only by adding tetraoctyl ammonium bromide(TOAB)(1.2 eq.to HAuCl4),changing the concentration of HAuCl4(10 mM)and the ratio of dodecanethiol(C12SH)to HAuCl4(5∶1),Au25-nanocluster was synthesized at room temperature without any limit on the stirring speed.Comparison with the traditional two-phase method,the revised approach requires shorter reaction time,simpler operation and gives a higher productivity(60%).Comparison of the performance of ISE with other signal transducing materials,the MPCs based ISEs have shown better analytical properties.