Multiplexed detection of nucleic acids in a single inexpensive and easy-to-operate device is still in urgent need for practical POC diagnostics in resource-poor settings.A miniaturized,robust,multiplexed and point-of-care microcapillary-based loopmediated isothermal amplification(cLAMP)was presented in this report,for assaying nucleic acids.This assay integrates capillaries(glass or plastic)to introduce and house sample/reagents,segments of water droplets to prevent contamination,pocket warmers to provide heat,and a hand-held flashlight for a visual readout of the fluorescent signal.This cLAMP system overcomes the problem of contamination in conventional LAMP and achieves a high sensitivity of two copies of standard plasmid.Two RNA targets of human immunodeficiency virus(HIV)from multiple plasma samples were detected simultaneously in the cLAMP system.As few nucleic acid detection methods can be wholly independent of external power supply and equipment,our cLAMP holds great promise for point-of-care applications in resourcepoor settings.