Isolation of Endogenously Assembled RNA-Protein Complexes Using Affinity Purification Based on Strep

来源 :中华医学会2015年医学病毒学大会 | 被引量 : 0次 | 上传用户:donnybaby
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
  Efficient isolation of endogenously assembled viral RNA-protein complexes is essential for understanding virus replication mechanisms.We have developed an affinity purification strategy based on an RNA affinity tag that allows large scale preparation of native viral RNA-binding proteins(RBPs).
其他文献
研究背景:人巨细胞病毒(HCMV,Human cytomegalovirus)侵入上皮细胞或内皮细胞与侵入成纤维细胞在机制上有明显的不同;在感染上皮细胞和内皮细胞的穿入阶段,要求特殊五联体复合物(PC,pentamericcomplex)的参与,其PC 由 gH/gL/UL128/UL130/UL131 构成。
研究背景卡波氏肉瘤相关疱疹病毒(Kaposi’s sarcoma associated herpesvirus,KSHV),又名人类疱疹病毒8 型(Human Herpesvirus 8,HHV8),属γ 疱疹病毒亚科,是卡波氏肉瘤的致病病原体。
目的:本研究通过荧光载体构建及荧光素酶报告基因鉴定miR-UL36-5p 与ANT3 mRNA 3’UTR 的结合能力。通过体外转染与western blot 检测miR-UL36-5p 在三种细胞系(HEK293,HELF 和U373 细胞系)中对ANT3 在蛋白水平的调控。采用流式细胞检测的方法,检测miR-UL36-5p 通过对ANT3 的调控对细胞生物功能的影响。
Objective To explore the detection of HCMV pp67 mRNA in peripheral blood by RT-PCR for the diagnosis of HCMV active infection.Methods the peripheral blood samples of 86 postoperative cancer patients w
水痘带状疱疹病毒(varicella-zoster virus,VZV)是一种高传染性病毒,初次感染儿童后主要引起水痘,一般在儿童中表现为自限性,症状较轻。但是,有少量残存的病毒会终身潜伏于宿主的神经节的神经细胞中,在成人中病毒的再激活感染会引起带状疱疹和各种不同的神经并发症,如脑膜炎和脊髓炎。
会议
Congenital human cytomegalovirus(HCMV)infection is the leading cause of neurological disabilities in newborns.Notch signaling pathway has been shown to be dysregulated by HCMV infection inhuman neural
会议
Congenital human cytomegalovirus(HCMV)infection is the leading cause of birth defects and manifests primarily as neurological disabilities.The mechanisms underlying HCMV-associated neurodevelopmental
会议
Previous studies have shown that neural progenitor cells(NPCs)are fully permissive for human cytomegalovirus(HCMV)infection and that infection results in neural cell loss and premature NPC differentia
会议
Objective: To study whether the dsRNA exist during Hantaan virus infection.Methods: J2 double-stranded RNA(dsRNA)antibody was employed to stain thedsRNA in Hantaan virus(HTNV)and Japanese encephalitis
会议
背景甲型流行性感冒病毒(influenza A virus,IAV)是流行性感冒的病原体.IAVRNA 共编码10 种蛋白质,其中NS1、NS2 为非结构蛋白.这两种蛋白质在病毒转录以及感染过程中都起到了关键性的作用,与病毒的致病性密切相关.