【摘 要】
：
2-aminopurine has been used as a fluorescent analogue of adenine for many years.Its fluorescence is strongly quenched when incorporated within DNA and pertu
【机 构】
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School of Chemistry and the Collaborative Optical Spectroscopy, Micromanipulation and lmaging Centre
【出 处】
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2007 Enzyme Engineering Conference(国际酶工程学术会议)
论文部分内容阅读
2-aminopurine has been used as a fluorescent analogue of adenine for many years.Its fluorescence is strongly quenched when incorporated within DNA and perturbations such as base flipping can cause a dramatic enhancement of the emission.However,it has been found that not all methyltransferases cause such an enhancement even though base flipping should be occurring.Some enzymes even cause an enhancement when the 2-AP is placed at a site which should not undergo base flipping.
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