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In this research we use gas chromatography-mass spectrometry (GC-MS) to determine metabolites of toluene diisocyanate (TDI) in mice and deduce the pathway for toluene diisocyanate metabolism in the organism.Conditions for TDI chromatography: Supelco PTETM-5 chromatographic column (30 mm × 0.25 mm × 0.25 μm); initial column temperature: 90℃, which was maintained for 30 min, after which the temperature was increased at a rate of 40℃/min to 280℃, which temperature was then maintained for 5.25 min; temperature for the vaporizing chamber: 250℃; carrier gas: helium flowing at 1.0 mL/min.Conditions for chromatography of TDI metabolites in the organism: 94% methyl, 4% ethenyl-bonded-phase fused-silica capillary column [30(+2)m× 0.25 (+ 0.02) mm]; initial column temperature: 30℃, which was maintained for 5 min, after which the temperature was increased at a rate of 80℃/min to 280℃, which temperature was then maintained for 5 min; temperature for the vaporizing chamber: 250℃; cartier gas: helium flowing at 1.0 mL/min.Conditions for mass spectrometry: EI for ionization; 70 eV for ionization energy; 280℃ for connecting tube temperature; 35-350 amu for range of scanning; and 1.0 μL for sample size.The results showed 2,4-toluene diisocyanate was metabolized into 2,4-diaminotoluene.Under the conditions selected for GC-MS, TDI metabolites in the organism can be isolated and identified.