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Objective:We have undertaken a large-scale project investigating the genomic and phenotypic predictors of PRRS resistance in pregnant gilts.One of the objectives was to evaluate temporal changes in white blood cell counts (WBCs) and peripheral blood mononuclear cell (PBMC) subsets following infection to determine if any predict PRRS severity.Methods:At gestation day 85 (+1),114 gilts were inoculated with a Type 2 PRRSV strain,50% IM and 50% IN (105 TCID50 total dose).Nineteen gilts were similarly sham inoculated (control).All gilts were euthanized 21dpi and the fetal preservation recorded.Heparinized blood was collected on 0,2,6 and 19 dpi.Automated WBC counts (Cell-Dyn 3500,Abbott Diagnostics) and manual differential counts were performed (300 cells total).PBMC isolated from whole blood were stained with fluorochrome-conjugated,monoclonal antibodies to define cell subsets using flow cytometry.General estimating equations were used to relate fetal mortality to cell subsets.