【摘 要】
：
Lineage-specific microRNAs(miRNAs)may contribute to functions specific to hematophagous mosquitoes and,as such,have potential for contributing to the develo
【机 构】
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CollegeofPlantProtection,KeylaboratoryofEntomologyandPestcontrol,SouthwestUniversity,Chongqing,P.R.C
【出 处】
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The International Conference on Insect Molecular Ecology and
论文部分内容阅读
Lineage-specific microRNAs(miRNAs)may contribute to functions specific to hematophagous mosquitoes and,as such,have potential for contributing to the development of future mosquito control approaches.Here we report that the mosquito-and gut-specific miRNA,miR-1174,is required for proper sugar absorption,fluid excretion,blood intake,and,consequently,egg maturation and survival in female mosquitoes.miR-1174 is highly expressed and localized in the posterior midgut,the blood-digesting portion of the mosquito alimentary canal.Depletion of miR-1174 results in severe defects in sugar absorption and blood intake.We identified serine hydroxymethyltransferase(SHMT)is a direct miR-1174 target.The adverse phenotypes caused by miR-1174 silencing were rescued by SHMT RNA interference.Our results suggest that miR-1174 is essential for fine-tuning the SHMT transcript to levels necessary for normal mosquito gut functions.
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