Metallic nanoparticles with core-shell architecture have been used to increase the fluorescence intensity and improve the sensitivity of fluorescent detection[1].This metal-enhanced fluorescence (MEF) phenomenon can be generated when the fluorophore is localized at a distance of a few nanometers from the metal surface.Typically,the core-shell Ag@SiO2 nanoparticles (Ag@SiO2 NPs) have been reported for ultrasensitive MEF with numerous fluorophores[2].For high sensitive fluorescent detection in the microfluidic system,we designed a simple laser induced fluorescence (LIF) detection system based on MEF effect.Ag@SiO2 NPs were introduced to the inner surface of microchannels of poly(dimethylsiloxane) (PDMS) chip (Fig.1).During the microfluidic electrophoresis analysis,the interactions of the Ag@SiO2 NPs with dichlorofluorescein (DCF) result in fluorescence enhancement and increased photostability (Fig.2).