Leaf Spot Caused by Phaeoisariopsis griseola on Phaseolus vulgaris in Organic Greenhouses in Shandon

来源 :2012年中国菌物学会学术年会 | 被引量 : 0次 | 上传用户:guigui198302
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  Angular leaf spots of green bean (Phaseolus vulgaris L.) were observed in organic greenhouses in Qingdao.Leaf lesions were gray, irregular to angular, Lesions on petioles were long.Conidia in vivo were curved cylindrical to obclavate with one to four sepeta and measured 46.2-50.8μm ×7.6-8.0μm.The conidiophores were 219-222 μm high and clustered together to form synnemata measuring 41.4 to 44.7μm in diameter.The pathogen was isolated by placing conidia from diseased leaves onto potato dextrose agar (PDA).Colonies measuring 11-12 mm in diameter composed of dense, dark olive mycelium developed after incubation in the dark at 25℃ for 25 days.The causal agent was tentatively identified as Phaeoisariopsis griseola (Sacc.) Ferraris, the cause of angular leaf spot of bean, and molecular identification were done by rDNA-ITS.Pathogenicity of the isolate was tested on Phaseolus vulgaris and Vigna unguiculata with conidia obtained from the second subculture of 14-day-old colonies on PDA.Conidial suspensions of 2 × 104 conidia per ml were sprayed onto the upper and lower surfaces of the first trifoliolate leaves of six runner bean plants, 18 days after planting.Inoculated and control plants (sprayed with distilled water) were placed in a growth chamber with a 12-h photoperiod at 24 ± 2℃ and 95 to 100% relative humidity and 48 h later moved to the greenhouse.Disease symptoms were evaluated 18days after inoculation.While control plants were healthy, all inoculated plants showed symptoms similar to those observed in the field.The fungus that was consistently reisolated from lesions in the inoculated plants was identified as Phaeoisariopsis griseola on the basis of fungal morphology, symptoms produced on leaves.To our knowledge, even thousgh there are many report of this disease in other provinces of China, this is the first report of angular leaf spot occurring on P.vulgaris in Shandong, and its pathogenicity on Vigna unguiculata indicated that Phaeoisariopsis griseola is a potential threat to Leguminosae plants.
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