【摘 要】
：
烟草抗病突变株SN01、SN02为本实验室筛选获得的高抗PVYN (potato virus Y vein necrosis strain)的具有自主知识产权的烟草新种质.为了进一步明确其抗PVYN的分子机制,本文研究其抗病相关基因,并分析抗感烟株中该基因的表达量差异情况.首先通过比较GenBank 中不同植物抗PVY基因序列,根据保守区设计引物,采用RT-PCR技术从抗病突变株SN01和SN02
【机 构】
：
沈阳农业大学植物保护学院,沈阳 110866 沈阳农业大学植物保护学院,沈阳 110866;内蒙古
【出 处】
：
中国植物病理学会2014年学术年会
论文部分内容阅读
烟草抗病突变株SN01、SN02为本实验室筛选获得的高抗PVYN (potato virus Y vein necrosis strain)的具有自主知识产权的烟草新种质.为了进一步明确其抗PVYN的分子机制,本文研究其抗病相关基因,并分析抗感烟株中该基因的表达量差异情况.首先通过比较GenBank 中不同植物抗PVY基因序列,根据保守区设计引物,采用RT-PCR技术从抗病突变株SN01和SN02及其对应感病亲本NC89和K326中成功克隆获得了真核生物翻译起始因子4E (eIF4E)全长基因,并分别命名为eIF4E01、eIF4E02、eIF4E89和eIF4E26,其开放阅读框ORF长度均为669bp,编码222个氨基酸.将eIF4E01、eIF4E02提交到GENEBANK得到登录号GenBank：KJ143694和GenBank： KJ184848.
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