Botrytis cinerea, an important plant pathogenic fungus, causes severe disease in a wide range of plant species both in the field and postharvest situations and results in significant economic losses.The molecular and cellular mechanisms involved in plant resistance to Botrytis and their genetic control have poorly been understood up to now.Arabidopsis is a small flowering plant and has been widely used as a model organism for researching the cellular and molecular interaction between fungi and plants.It was found that different Arabidopsis ecotypes appeared different responses while being infected by Botrytis in our preliminary studies, for example, Col-0 eco(i)ype appeared immunity, and Ws-0 ecotype appeared HR response, while Ler ecotype appeared susceptible symptoms.In this paper, we have isolated one resistant-related gene of Arabidopsis against Botrytis by DDRT-PCR (differential display reverse transcription-PCR) and RACE (Rapid amplification of cDNA ends).The resistant-related gene was identified as AT5G67480 gene that encodes binding protein or transcription regulator.AT5G67480 gene was identified as a single copy in Arabidopsis genome by PCR and Southern analysis and it locates between 26948165 bp and 26950151 bp on chromosome Ⅴ of Arabidopsis Col-0 ecotype.