Rice stripe disease (RSD),caused by rice stripe virus (RSV) is one of the most serious diseases in temperate rice-growing areas.In the present study,we created an RNAi construct containing coat protein gene (CP) and disease specific protein gene (SP) sequences from RSV.The RNAi construct was transformed into two susceptible japonica varieties,Suyunuo (SYN) and Guanglingxiangjing (GLXJ),to develop resistance against RSV.